Analysis Of T Cell Clonality Using Laser Capture Microdissection And

Analysis Of T Cell Clonality Using Laser Capture Microdissection And Here, we assessed the diagnostic utility of laser capture microdissection (lcm) and high resolution microcapillary electrophoresis in the analysis of clonality of small biopsy specimens. clonality was determined in 24 cases: five reactive tonsils, five reactive lymph nodes, six inflammatory skin lesions, and eight t cell lymphomas. Identification of a clonal lymphocytic population may be difficult because of the paucity of the infiltrate as well as a heterogeneous background population of cells. employment of laser capture microdissection (lcm) enables isolation of target t lymphocytes from other lymphoid cells in tissue samples. 12 13 14 this methodology has been applied.

Pdf Analysis Of T Cell Clonality Using Laser Capture M Identification of clonal lymphocytic populations by polymerase chain reaction may be difficult in cases with scant cellular infiltrates or those with a heterogeneous population of cells. here, we assessed the diagnostic utility of laser capture microdissection (lcm) and high resolution microcapillary electrophoresis in the analysis of clonality of small biopsy specimens. clonality was. Employment of laser capture microdissection (lcm) enables isolation of target t lymphocytes from other lymphoid cells in tissue samples.12,13,14 this methodology has been applied for molecular analysis of composite lymphomas, where two unrelated clonal lymphocytic populations occupy different but intimately interwoven microenvironments.15 in. Diagnostic utility of laser capture microdissection (lcm) and high resolution microcapillary electro phoresis in the analysis of clonality of small biopsy specimens. clonality was determined in 24 cases: five reactive tonsils, five reactive lymph nodes, six inflammatory skin lesions, and eight t cell lympho mas. Yakirevich et al. demonstrated the diagnostic utility of high resolution microcapillary electrophoresis for clonality analysis in small biopsy specimens from laser capture microdissection [12]. this technique allowed detection of as few as 2% of clonal t cells in a mixed lymphocytic infiltrate and of 0.1% in a mixture of non lymphoid dna.