Follicle Stem Cell Lineage Intracellular Ph Imaging Protocol Preview Here, we describe a protocol that we routinely use for imaging live drosophila ovarioles to measure phi in the epithelial follicle stem cell (fsc) lineage in mcherry::phluorin transgenic wild type lines; however, the methods described here can be easily adapted for other tissues, including the wing discs and eye epithelium. We provide a protocol for imaging intracellular ph of an epithelial stem cell lineage in live drosophila ovarian tissue. we describe methods to generate transgenic flies expressing a ph biosensor, mcherry::phluorin, image the biosensor using quantitative fluorescence imaging, generate standard curves, and convert fluorescence intensity values to ph values.
Methods For Imaging Intracellular Ph Of The Follicle Stem Cell о Watch this scientific journal video about methods for imaging intracellular ph of the follicle stem cell lineage in live drosophila ovarian tissue at jove. Sumitra tatapudy, marimar benitez, todd nystul. methods for imaging intracellular ph of the follicle stem cell lineage in live <em>drosophila< em> ovarian tissue. Here, we describe a protocol that we routinely use for imaging live drosophila ovarioles to measure phi in the epithelial follicle stem cell (fsc) lineage in mcherry::phluorin transgenic wild type. Watch the full video at jove v 56316 methods for imaging intracellular ph follicle stem cell lineage live?utm source= &utm medium=socia.
Methods For Imaging Intracellular Ph Of The Follicle Stem Cell о Here, we describe a protocol that we routinely use for imaging live drosophila ovarioles to measure phi in the epithelial follicle stem cell (fsc) lineage in mcherry::phluorin transgenic wild type. Watch the full video at jove v 56316 methods for imaging intracellular ph follicle stem cell lineage live?utm source= &utm medium=socia. Here, we describe a protocol that we routinely use for imaging live drosophila ovarioles to measure phi in the epithelial follicle stem cell (fsc) lineage in mcherry::phluorin transgenic wild type lines; however, the methods described here can be easily adapted for other tissues, including the wing discs and eye epithelium. we describe. Request pdf | on sep 26, 2017, sumitra tatapudy and others published methods for imaging intracellular ph of the follicle stem cell lineage in live drosophila ovarian tissue | find, read and cite.
Methods For Imaging Intracellular Ph Of The Follicle Stem Cell о Here, we describe a protocol that we routinely use for imaging live drosophila ovarioles to measure phi in the epithelial follicle stem cell (fsc) lineage in mcherry::phluorin transgenic wild type lines; however, the methods described here can be easily adapted for other tissues, including the wing discs and eye epithelium. we describe. Request pdf | on sep 26, 2017, sumitra tatapudy and others published methods for imaging intracellular ph of the follicle stem cell lineage in live drosophila ovarian tissue | find, read and cite.
Methods For Imaging Intracellular Ph Of The Follicle Stem Cell о
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