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Furthermore, specify a scoring matrix for BLASTP, BLASTX, TBLASTN and TBLASTX. The default matrix is BLOSUM62. The valid alternative choices include PAM30, PAM70, BLOSUM45 and BLOSUM80. The matrix parameter is ignored for BLASTN nucleotide searches. For DNA sequences, DNA substitution matrices are used instead. This aspect of Welcome To Blast Help Blasthelp Documentation plays a vital role in practical applications.

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Furthermore, documentation and sample perl code are available. This is a public resource, so usage limitations apply. Projects that involve a large number of BLAST searches should use the RESTful interface at a cloud provider or stand-alone BLAST. Usage Guidelines The NCBI BLAST servers are a shared resource. We give priority to interactive users. This aspect of Welcome To Blast Help Blasthelp Documentation plays a vital role in practical applications.

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Furthermore, you can expand a cluster on your BLAST results to view and download a report or the sequences of all member proteins, and you can also perform a BLAST alignment of all the members of the cluster. This aspect of Welcome To Blast Help Blasthelp Documentation plays a vital role in practical applications.

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Furthermore, specify a scoring matrix for BLASTP, BLASTX, TBLASTN and TBLASTX. The default matrix is BLOSUM62. The valid alternative choices include PAM30, PAM70, BLOSUM45 and BLOSUM80. The matrix parameter is ignored for BLASTN nucleotide searches. For DNA sequences, DNA substitution matrices are used instead. This aspect of Welcome To Blast Help Blasthelp Documentation plays a vital role in practical applications.

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